The Prolific clone serial adapter has a few driver issues under Win10 - see:
I'm using a Prolific PL2303 (clone) USBSerial adaptor rather than a DE9 M-F cable as originally supplied, as I don't currently have a working legacy COM port to plug it into! Unfortunately it didn't come with any cables, and as I didn't have any IDC connectors in the right sizes, I spent an evening crimping about 70 female duPont contacts to make up 6' replacements for the 2x 10 way and 1x 6 way IDC cables and 4x 2way jumper cables it originally was sold with (See TME STK500 page). HV serial programming with an external clock, or even HV parallel programming, to recover an Atmel AVR family MCU that you've been dumb enough to set fuses for that it cant run with! IMHO its worth resurrecting the old STK500 even though I've got a choice of more modern USB AVR programmers, as its one of the few programmers that can do 'fuse-busting' i.e.
Thanks to another member, I recently was given a 2006 vintage Atmel STK500 AVR development board/programmer (originally released in early 2001), with a few AVR MCUs thrown in as well. Resurrecting an Atmel STK500 My Atmel STK500 AVR development board/programmer 3 Installing the WINAVR C compiler for use in AVR Studio 4.2 Installing Atmel AVR Studio 4 on Win10 圆4.